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Sort fish into respective species outside and count the number of each (roughly) Based on proportions of each species present decide on the number of each to sample Bring the fish inside Wearing sterile disposable gloves, uggs boots 5803 cheap sand colour womens lay 10 (depending on fish size) fish on a stainless steel dissecting tray overlaid with paper towel. Place round fish with head to the left and flat fish eyes up (will ugg boots 5804 cheap US 7 depend on if left or right eyed) Complete the sample record sheet Using 70% ethanol solution and paper towel surface sterilize each fish Using a sterile disposable scalpel cut open the body and cranial cavities of each fish in the pool (scalpel 1) Change gloves and scalpel (scalpel 2) Open the body cavity of the first fish using a gloved hand, remove samples of spleen, kidney and heart tissue and place ugg boots cheap uk in a labelled VTM tube (scalpel 2) Repeat for each fish in the pool Using the same scalpel (scalpel 2) remove a sample of brain tissue and place in the same labelled VTM tube Repeat for each fish in the pool Note: Final tissue volumes in each pool must be between the minimum and maximum marks on the virus reference tubes Used scalpels and forceps go into a sharps container All sampled fish are to be double bagged in heavy duty black bin liners Used towel roll and gloves go into a separate black bin liner Clean the tray with a solution of 70% ethanol The labelled VTM tube goes into a stainless steel rack contained within a polystyrene cool box and freezer blocks When the bags are full, or at the end of the sampling day, they must be put into the freezer adjacent to the laboratory and labelled as to the contents. Additional chaussure jordan son of mars pas cher wholesale uggs 5808 from factory.

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